2013 International Workshop on Electrical Penetration Graph (EPG)
Research Methods for Study of Piercing-sucking Arthropod Feeding
July 21 – 27, 2013
Electrical penetration graph (EPG) monitoring is a powerful research technique for the study of feeding by piercing-sucking arthropod pests such as aphids, whiteflies, leafhoppers, plant bugs, mosquitoes and ticks. Like electrocardiography (EKG) in medical studies, EPG produces an electrical waveform that can be interpreted to identify all the feeding behaviors performed while the insects’ mouthparts are inserted, otherwise invisibly, inside a plant. Participants will be trained primarily with the newest the AC-DC monitor, but older monitor types, such as the DC monitor, will also be demonstrated and discussed.
There is a strong need for this technique in biological and agricultural research today. For example, EPG is being used to develop new crop varieties resistant to damage by such agricultural pests, including such exotic, invasive species as Asian citrus psyllid, brown marmorated stink bug, soybean aphid, brown citrus aphid, glassy-winged sharpshooter, potato psyllid, and many others.What can you do with skills in EPG?
Biologists skilled in this methodology are employable in many university and government research facilities, worldwide. In addition, the technique is applicable to many types of graduate research in biological or agricultural sciences. The workshop instructors can help brainstorm research topics and ideas for graduate students who attend the course.Objectives of the Workshop
To teach a researcher in entomology/biology all methods necessary to perform independent research with the new AC-DC monitor, including:
- Wiring aphids, leafhoppers, and other insects
- Recording and measuring EPG waveforms
- Statistical analysis of waveform data using new, state-of-the-art SAS programs for both sequential and non-sequential variables (Backus et al. 2007, Sarria et al. 2009).
- Background information on electronics, principles of EPG, hemipteran feeding behavior
- Discussion of complementary methods to use with EPG, e.g. histology, artificial diets (no demonstrations)
Experienced EPG Research Are Encouraged to Enroll, As Well as Novices
The EPG Workshop offers valuable information for both novices and experienced, advanced EPG researchers. Click here for details of the program tailored to experienced researchers.
Visas and Visa Waivers
International visitors coming to the United States will need a Visa or a Visa Waiver, depending on the country of residence.
For international visitors traveling to Fresno for the EPG Monitoring of Insect Feeding workshop, most will need to obtain a B-1 (Business 1) Visa. See more information about the B1/B2 Visa: http://travel.state.gov/visa/temp/types/types_1265.html
On the other hand, if the visitors are from a country recognized as a Visa Waiver program country, they will instead obtain a Visa Waiver by going to the ETSA site: https://esta.cbp.dhs.gov/esta/ See more information about the Visa Waiver program: http://travel.state.gov/visa/temp/types/types_1265.html
Click here to download a flyer and registration form.
Click here to download a detailed brochure.
Click here to download the scholarship application instructions.
Click here to download a sample curriculum vita.
Enrollment will be limited to 36 participants, so sign up today!
Download Optional Readings
- Backus & Bennett 2009 AC-DC mon.pdf
- Petterson, Tjallingii & Hardie 2007 aphids as crop pests review.pdf
- Wayadande & Nault 1993 leafhopper X waves.pdf
- Backus et al. 2009 SS X wave.pdf
- Dugravot, Backus, Reardon & Miller 2008 cib EMG.pdf
- Joost, Backus et al 2006 stylet acts.pdf
- 3 Tjallingii, W.F. 2000. Comparison of AC and DC system. (book Section chapter 3).pdf
- Almeida & Backus 2004 BGSS.pdf
- Backus et al. 2000 comp signal processing.pdf
- Backus et al. GWSS wf corr 2005.pdf
- Backus et al. 2012 GWSS saliva.pdf
- Serikawa et al 2012 ACP feeding on imidacloprid.pdf